Journal of agricultural and food chemistry, 2012-09, Vol.60 (36), p.9055-9061
2012
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Grape Seed-Derived Procyanidins Decrease Dipeptidyl-peptidase 4 Activity and Expression
- Ist Teil von
- Journal of agricultural and food chemistry, 2012-09, Vol.60 (36), p.9055-9061
- Ort / Verlag
- Washington, DC: American Chemical Society
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Dipeptidyl-peptidase 4 (DPP4) inhibitors are among the newest treatments against type 2 diabetes. Since some flavonoids modulate DPP4 activity, we evaluated whether grape seed-derived procyanidins (GSPEs), which are antihyperglycemic, modulate DPP4 activity and/or expression. In vitro inhibition assays showed that GSPEs inhibit pure DPP4. Chronic GSPE treatments in intestinal human cells (Caco-2) showed a decrease of DPP4 activity and gene expression. GSPE was also assayed in vivo. Intestinal but not plasmatic DPP4 activity and gene expression were decreased by GSPE in healthy and diet-induced obese animals. Healthy rats also showed glycemia improvement after oral glucose consumption but not after an intraperitoneal glucose challenge. In genetically obese rats, only DPP4 gene expression was down-regulated. Thus, procyanidin inhibition of intestinal DPP4 activity, either directly and/or via gene expression down-regulation, could be responsible for some of their effects in glucose homeostasis.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-8561eISSN: 1520-5118DOI: 10.1021/jf3010349Titel-ID: cdi_proquest_miscellaneous_1039346170
- Format
- –
- Schlagworte
- Animals, Biological and medical sciences, blood glucose, Blood Glucose - metabolism, Caco-2 Cells, Diabetes Mellitus, Type 2 - drug therapy, Diabetes Mellitus, Type 2 - enzymology, Diabetes Mellitus, Type 2 - genetics, Diabetes Mellitus, Type 2 - metabolism, Dipeptidyl Peptidase 4 - genetics, Dipeptidyl Peptidase 4 - metabolism, Down-Regulation - drug effects, Female, flavonoids, Food industries, Fruit and vegetable industries, Fundamental and applied biological sciences. Psychology, gene expression, Gene Expression - drug effects, gene expression regulation, glucose, Grape Seed Extract - pharmacology, grapes, homeostasis, Humans, noninsulin-dependent diabetes mellitus, Proanthocyanidins - pharmacology, procyanidins, Rats, Rats, Wistar, Rats, Zucker, Vitis - chemistry