Tuberculosis (Edinburgh, Scotland), 2012-09, Vol.92 (5), p.407-416
2012
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Details
- Autor(en) / Beteiligte
- Titel
- Rapid detection of Mycobacterium tuberculosis biomarkers in a sandwich immunoassay format using a waveguide-based optical biosensor
- Ist Teil von
- Tuberculosis (Edinburgh, Scotland), 2012-09, Vol.92 (5), p.407-416
- Ort / Verlag
- Scotland: Elsevier Ltd
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Summary Early diagnosis of active tuberculosis (TB) remains an elusive challenge, especially in individuals with disseminated TB and HIV co-infection. Recent studies have shown a promise for the direct detection of pathogen-specific biomarkers such as lipoarabinomannan (LAM) for the diagnosis of TB in HIV-positive individuals. Currently, traditional immunoassay platforms that suffer from poor sensitivity and high non-specific interactions are used for the detection of such biomarkers. In this manuscript, we demonstrate the development of sandwich immunoassays for the direct detection of three TB-specific biomarkers, namely LAM, early secretory antigenic target 6 (ESAT6) and antigen 85 complex (Ag85), using a waveguide-based optical biosensor platform. Combining detection within the evanescent field of a planar optical waveguide with functional surfaces that reduce non-specific interactions allows for the ultra-sensitive and quantitative detection of biomarkers (an order of magnitude enhanced sensitivity, as compared to plate-based ELISA) in complex patient samples (urine, serum) within a short time. We also demonstrate the detection of LAM in urine from a small sample of subjects being treated for TB using this approach with excellent sensitivity and 100% corroboration with disease status. These results suggest that pathogen-specific biomarkers can be applied for the rapid and effective diagnosis of disease. It is likely that detection of a combination of biomarkers offers greater reliability of diagnosis, rather than detection of any single pathogen biomarker. NCT00341601.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1472-9792eISSN: 1873-281XDOI: 10.1016/j.tube.2012.05.009Titel-ID: cdi_proquest_miscellaneous_1038605095
- Format
- –
- Schlagworte
- Acyltransferases - metabolism, Antigen 85 complex, Antigens, Bacterial - metabolism, Bacterial Proteins - metabolism, biomarkers, Biomarkers - metabolism, Biosensing Techniques, Biosensors, Coinfection, Diagnosis, Early secretory antigenic target 6, Enzyme-Linked Immunosorbent Assay, Female, HIV Seropositivity - epidemiology, HIV Seropositivity - metabolism, Human immunodeficiency virus, Humans, Immunoassay, Immunoassays, Infectious Disease, Lipoarabinomannan, Lipoarabinomannans, Lipopolysaccharides - metabolism, Male, Mycobacterium tuberculosis, Mycobacterium tuberculosis - isolation & purification, Mycobacterium tuberculosis - pathogenicity, Pathogens, Pulmonary/Respiratory, Reagent Kits, Diagnostic, Reproducibility of Results, Sensitivity and Specificity, Tuberculosis, Tuberculosis, Pulmonary - diagnosis, Tuberculosis, Pulmonary - epidemiology, Tuberculosis, Pulmonary - metabolism, Urine, Waveguide-based optical biosensor