Details

Autor(en) / Beteiligte

• Li, Qiang
• Zhang, Kaihuan
• Wang, Tongzhou
• Zhou, Xinying
• Wang, Jia
• Wang, Chen
• Lin, Haixiao
• Li, Xin
• Lu, Ying
• Huang, Guoliang

Titel

Color-encoded microcarriers based on nano-silicon dioxide film for multiplexed immunoassays

Ist Teil von

• Analyst (London), 2012-08, Vol.137 (16), p.3760-3766

Ort / Verlag

Cambridge: Royal Society of Chemistry

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Multiplexed analysis allows researchers to obtain high-density information with minimal assay time, sample volume and cost. Currently, microcarrier or particle-based approaches for multiplexed analysis involve complicated or expensive encoding and decoding processes. In this paper, a novel optical encoding technique based on nano-silicon dioxide film is presented. Microcarriers composed of thermally grown silicon dioxide (SiO(2)) film and monocrystalline silicon (Si) substrate were fabricated. The nano-silicon dioxide film exhibited unique surface color by low-coherence interference. Hence the colors can be used for encoding at least 100 microcarriers loaded with films of different thickness. We demonstrated that color-encoded microcarriers loaded with antigens could be used for multiplexed immunoassays to detect goat anti-human IgG, goat anti-mouse IgG and goat anti-rabbit IgG, with fluorescent detection as the interrogating approach. This microcarrier-based method also exhibited improved analytical performance compared with a microarray technique. This approach will provide new opportunities for multiplexed target assay development.