Details

Autor(en) / Beteiligte

• Yamada, Parida
• Hatta, Tamao
• Du, Mingyuan
• Wakimizu, Kenji
• Han, Junkyu
• Maki, Taichi
• Isoda, Hiroko

Titel

Inflammatory and degranulation effect of yellow sand on RBL-2H3 cells in relation to chemical and biological constituents

Ist Teil von

• Ecotoxicology and environmental safety, 2012-10, Vol.84, p.9-17

Ort / Verlag

San Diego, CA: Elsevier Inc

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Recent studie pointed out that allergic diseases have increased during the Asian dust storm event (ADSE) in Japan. Daily observations and the atmospheric concentrations of yellow sand (YS) aerosol have been increasing. In this study, YS samples collected from three sites of Japan during ADSE in 2009–2010 were used. The particles were analyzed by X-ray photoelectron spectroscopy (XPS) and X-ray fluorescence—energy dispersive spectrometer (XRF-EDS). We investigate ability of YS extract on enhancing the chemical mediator release and cytokine production from rat basophilic leukemia (RBL-2H3) cells. The dust particles at Fukuoka and Tsukuba were abundant in aluminum (Al), iron (Fe), potassium (K) and titan (Ti) than those at Naha. Concentration of the trace endotoxin and Cryptomeria japonica pollen allergen (Cry j 1) were measured in YS extract. After exposure of RBL-2H3 cells to YS extract, the β-hexosaminidase (β-hex) release, tumor necrosis factor-alpha (TNF-α) production were enhanced in RBL-2H3 cells. This process depends on endotoxin, Cry j 1 and other allergen present in the YS extract. YS water extract also show a strong cytotoxic effect on the cells. This data suggest that low levels of endotoxin and Cry j 1 in YS may cause allergy during the ADSE. ► The allergen activity of YS on basophilic cells was investigated. ► YS extract collected from Japan can enhance β-hex release by basophilic cells. ► This process depends on endotoxin, Cry j 1, and other allergen content. ► TS water extract shows the strong cytotoxic effect on basophilic cells. ► The low level of LPS and Cry j1 in YS may cause allergy during the ADSE.