Details

Autor(en) / Beteiligte

• Malartre, Nicolas
• Boulieu, Roselyne
• Falah, Nisrine
• Cortay, Jean-Claude
• Lina, Bruno
• Morfin, Florence
• Frobert, Emilie

Titel

Effects of mutations on herpes simplex virus 1 thymidine kinase functionality: An in vitro assay based on detection of monophosphate forms of acyclovir and thymidine using HPLC/DAD

Ist Teil von

• Antiviral research, 2012-09, Vol.95 (3), p.224-228

Ort / Verlag

Kidlington: Elsevier B.V

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• ► A non-isotopic method to assess TK activity using ACV and dT as substrates. ► Dosage of monophosphate forms of both ACV and dT using HPLC/DAD. ► Phenotypes of TKs characterized as TK altered, TK deficient or TK partial. ► Database of UL23 TK gene completed with six novel mutations. ► Importance for fast and efficient HSV genotyping. Discrimination between the mutations responsible for drug resistance and those of UL23 TK gene polymorphism can be difficult. A non-isotopic method has been developed to assess TK functionality by measuring monophosphate forms of both acyclovir (ACV) and thymidine using HPLC/DAD. Phenotypes of TKs could thus be characterized as TK altered (P84L, A189V, L227F), TK deficient (G200S, L291P) or TK partial (R163H). A reliable link between HSV UL23 TK mutations and ACV resistance is necessary for developing a powerful genotyping tool to detect ACV resistance quickly in clinical samples.