Zhong hua yi xue za zhi, 2012-05, Vol.92 (18), p.1240-1243
2012
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- Autor(en) / Beteiligte
- Titel
- Expression of lymphokines in CD8(+)HLA-DR(+) T lymphocytes of patients with severe aplastic anemia
- Ist Teil von
- Zhong hua yi xue za zhi, 2012-05, Vol.92 (18), p.1240-1243
- Ort / Verlag
- China
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- To examine the expression of lymphokines damaging hematopoietic cells by CD8(+)HLA-DR(+) effector T cells in peripheral blood (PB) of the patients with severe aplastic anemia (SAA) and explore further the heterogeneous immunopathogenesis of SAA. The CD8(+)HLA-DR(+) cells were sorted by immunomagnetic separation from the PB of 24 untreated SAA patients and 23 normal controls. The mRNA expressions of perforin, granzyme B, FasL and tumor necrosis factor β (TNF-β) of sorted cells were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). The mRNA levels of perforin, granzyme B of CD8(+)HLA-DR(+)T cells in untreated group were higher than those of the controls (0.66 ± 0.25, 0.56 ± 0.26 vs 0.53 ± 0.14, 0.40 ± 0.13, P = 0.042, 0.012). The mRNA level of FasL in CD8(+)HLA-DR(+) T cells of untreated SAA patients was higher than that of the controls (0.77 ± 0.24 vs 0.61 ± 0.16, P = 0.011). The mRNA of TNF-β in CD8(+)HLA-DR(+) T cells of untreated SAA patients was also higher than that of the controls (0.
- Sprache
- Chinesisch
- Identifikatoren
- ISSN: 0376-2491DOI: 10.3760/cma.j.issn.0376-2491.2012.18.006Titel-ID: cdi_proquest_miscellaneous_1033535599
- Format
- –
- Schlagworte
- Adolescent, Adult, Anemia, Aplastic - metabolism, Case-Control Studies, CD8-Positive T-Lymphocytes - immunology, CD8-Positive T-Lymphocytes - metabolism, Child, Female, Granzymes - metabolism, HLA-DR Antigens - immunology, Humans, Lymphokines - metabolism, Lymphotoxin-alpha - metabolism, Male, Perforin - metabolism, RNA, Messenger - genetics, Young Adult