Details

Autor(en) / Beteiligte

• GU, Wen Peng
• WANG, Xin
• QIU, Hai Yan
• LUO, Xia
• XIAO, Yu Chun
• TANG, Liu Ying
• KAN, Biao
• XU, Jian Guo
• JING, Huai Qi

Titel

Comparison of Lipopolysaccharide and Protein Immunogens from Pathogenic Yersinia enterocolitica Bio-serotype 1B/O：8 and 2/O：9 using SDS-PAGE

Ist Teil von

• Biomedical and environmental sciences, 2012-06, Vol.25 (3), p.282-290

Ort / Verlag

China: Elsevier B.V

Erscheinungsjahr

2012

Quelle

Elektronische Zeitschriftenbibliothek

Beschreibungen/Notizen

• Objective Yersinia enterocolitica is an extracellular pathogen and its related antigens interact with the host immune system. We investigated the difference in immunological characteristics between a highly pathogenic and poorly pathogenic strain of Y. enterocolitico. Methods We used SDS-PAGE and western blotting to characterize lipopolysaccharide （LPS）, Yersinio outer membrane proteins （Yops）, membrane proteins, and whole-cell proteins from poorly pathogenic Y. enterocolitico bio-serotype 2/0：9, isolated from China, and highly pathogenic bio-serotype 1B/O：8, isolated from Japan. Results These two strains of Y. enterocolitica had different LPS immune response patterns. Comparison of their Yops also showed differences that could have accounted for their differences in pathogenicity. The membrane and whole-cell proteins of both strains were similar; immunoblottting showed that the 35 kD and perhaps the 10 kD proteins were immunogens in both strains. Conclusion The major antigens of the two strains e and membrane proteins, as shown by comparing preparations. citing the host immune protein samples with response were the LPS reference and purified