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High growth rate, photosynthesis rate and increased hydrogen(ases) in manganese deprived cells of a newly isolated Nostoc-like cyanobacterium (SAG 2306)
Ist Teil von
International journal of hydrogen energy, 2011-09, Vol.36 (19), p.12200-12210
Ort / Verlag
Kidlington: Elsevier Ltd
Erscheinungsjahr
2011
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
A
Nostoc-like cyanobacterium, strain SAG 2306, was isolated from a clay soil sample at Assiut region (Egypt). Morphology and analysis of 16S rDNA identified it as a member of the heterocyteous cyanobacteria (Nostocales). Unexpectedly, chlorophyll
a and dry weight were higher in manganese deprived (Mn-) or in manganese double deprived (Mn--) than in replete (Mn+) cells. The latter phase of Mn-- diluted the cellular content in daughter cells to a critical minimal value of about 1.5% relative to the original 100% in Mn+. Similar to growth,
Nostoc sp. exhibited a significant age-dependent biphasic impact on hydrogenases activity. Older cells of 120 h age displayed higher hydrogen evolution rates than younger ones. The results suggest inhibitory effects of the 12 μM manganese contained in BG11 medium (Mn+) not only on growth but also on hydrogen production during the first 72 h of growth.
► Hydrogen evolution and hydrogenases of
Nostoc were enhanced by manganese deprivation. ► Enhanced hydrogenases may substitute inhibited MnSOD in scavenging free radicals. ► Inhibition of oxygen evolution by Mn deprivation facilitated installing anaerobiosis. ► Mn in BG11 (12 μM) medium inhibited
Nostoc growth and hydrogen(ases) at early stages. ► Growth in Mn deprived medium severely decreased the calculated cellular Mn to 0.8%.