Details

Autor(en) / Beteiligte

• Zhong, J.Z.
• Liu, W.
• Liu, C.M.
• Wang, Q.H.
• Li, T.
• Tu, Z.C.
• Luo, S.J.
• Cai, X.F.
• Xu, Y.J.

Titel

Aggregation and conformational changes of bovine β-lactoglobulin subjected to dynamic high-pressure microfluidization in relation to antigenicity

Ist Teil von

• Journal of dairy science, 2012-08, Vol.95 (8), p.4237-4245

Ort / Verlag

New York, NY: Elsevier Inc

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• Our previous research indicated that dynamic high-pressure microfluidization (DHPM) had a significant effect on the antigenicity of β-lactoglobulin (β-LG). In this study, aggregation and conformational changes subjected to DHPM (0.1–160MPa) were investigated in relation to antigenicity. When DHPM pressure increased from 0.1 to 80MPa, disaggregation of β-LG samples and partial unfolding of the molecule were accompanied by an increase in β-LG antigenicity, which was reflected in the decrease of particle size, increase of free sulfhydryl (SH) contents and β-strands contents, and slight exposure of aromatic amino acid residues. At pressures above 80MPa, the reaggregation of β-LG may contribute to the decrease in antigenicity, which was reflected by an increase in particle size, the formation of aggregates, a decrease of in SH and β-strands contents, and slight changes in aromatic amino acid residues. Aggregation and conformational changes of β-LG under DHPM was related to its antigenicity.