Details

Autor(en) / Beteiligte

• Studt, L.
• Troncoso, C.
• Gong, F.
• Hedden, P.
• Toomajian, C.
• Leslie, J.F.
• Humpf, H.-U.
• Rojas, M.C.
• Tudzynski, B.

Titel

Segregation of secondary metabolite biosynthesis in hybrids of Fusarium fujikuroi and Fusarium proliferatum

Ist Teil von

• Fungal genetics and biology, 2012-07, Vol.49 (7), p.567-577

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2012

Quelle

MEDLINE

Beschreibungen/Notizen

• ► We analyzed an interspecific cross between Fusarium fujikuroi and Fusarium proliferatum. ► We studied the production of gibberellins, fumonisins and fusarins in the progeny. ► The segregation pattern were far distant from the expected 1:1 Mendelian ratio. ► Among the progeny are isolates with a new composition of secondary metabolites. Fusarium fujikuroi and Fusarium proliferatum are two phylogenetically closely related species of the Gibberella fujikuroi species complex (GFC). In some cases, strains of these species can cross and produce a few ascospores. In this study, we analyzed 26 single ascospore isolates of an interspecific cross between F. fujikuroi C1995 and F. proliferatum D4854 for their ability to produce four secondary metabolites: gibberellins (GAs), the mycotoxins fusarin C and fumonisin B1, and a family of red polyketides, the fusarubins. Both parental strains contain the biosynthetic genes for all four metabolites, but differ in their ability to produce these metabolites under certain conditions. F. fujikuroi C1995 produces GAs and fusarins, while F. proliferatum D4854 produces fumonisins and fusarubins. The segregation amongst the progeny of these traits is not the expected 1:1 Mendelian ratio. Only eight, six, three and three progeny, respectively, produce GAs, fusarins, fumonisin B1 and fusarubins in amounts similar to those synthesized by the producing parental strain. Beside the eight highly GA3-producing progeny, some of the progeny produce small amounts of GAs, predominantly GA1, although these strains contain the GA gene cluster of the non-GA-producing F. proliferatum parental strain. Some progeny had recombinant secondary metabolite profiles under the conditions examined indicating that interspecific crosses can yield secondary metabolite production profiles that are atypical of the parent species.