Journal of dairy science, 2012-07, Vol.95 (7), p.3711-3721
2012
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- Autor(en) / Beteiligte
- Titel
- Genetic merit for fertility traits in Holstein cows: III. Hepatic expression of somatotropic axis genes during pregnancy and lactation
- Ist Teil von
- Journal of dairy science, 2012-07, Vol.95 (7), p.3711-3721
- Ort / Verlag
- New York, NY: Elsevier Inc
- Erscheinungsjahr
- 2012
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The objective of this study was to characterize the circulating concentrations of insulin-like growth factor-I (IGF-I) and the hepatic expression of key genes regulating the somatotropic axis in cows divergent in genetic merit for fertility traits but with similar genetic merit for milk production traits. A total of 11 cows with good genetic merit for fertility (Fert+) and 12 cows with poor genetic merit for fertility (Fert−) underwent liver biopsy by percutaneous punch technique on d 20 (±6.7 d) prepartum and on d 2 (±1.5 d), d 58 (±3.7 d), d 145 (±13 d), and d 245 (±17.1 d) postpartum. Total RNA was isolated and the mRNA expression of growth hormone receptor (GHR 1A and GHRtot), IGF-I, janus tyrosine kinase 2 (JAK2), signal transducer and activator of transcription 5B (STAT5B), suppressor of cytokine signaling 3 (SOCS-3), acid-labile subunit (ALS), and IGF-binding proteins (IGFBP1 to IGFBP6) were measured by real-time quantitative PCR. During lactation, the circulating concentrations of IGF-I were 34% greater in Fert+ cows. The Fert+ cows had increased mean expression of IGF-I mRNA during the study; however, the difference in IGF-I mRNA abundance between Fert+ and Fert− cows was most pronounced at d 145 and 245. The expression of IGFBP3 and ALS transcript was similar in Fert+ and Fert− cows for the duration of the study. The Fert− cows, however, had greater expression of IGFBP2, IGFBP4, IGFBP5, and IGFBP6. Genotype had no effect on mRNA abundance of GHR 1A, STAT5B, JAK2, or SOCS-3. Genetic merit for fertility traits affects hepatic expression of key genes of the somatotropic axis regulating the synthesis, bioavailability, and stability of circulating IGF-I.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-0302eISSN: 1525-3198DOI: 10.3168/jds.2011-4977Titel-ID: cdi_proquest_miscellaneous_1021980177
- Format
- –
- Schlagworte
- Animal productions, Animals, bioavailability, Biological and medical sciences, biopsy, Carrier Proteins - analysis, Carrier Proteins - genetics, Cattle - genetics, Cattle - physiology, cows, cytokines, Female, fertility, Fertility - genetics, Fertility - physiology, Food industries, Fundamental and applied biological sciences. Psychology, gene expression, genes, Genes - genetics, Genes - physiology, genetic merit, genetic merit for fertility, genotype, Glycoproteins - analysis, Glycoproteins - genetics, Holstein, insulin-like growth factor binding proteins, Insulin-Like Growth Factor Binding Proteins - analysis, Insulin-Like Growth Factor Binding Proteins - genetics, insulin-like growth factor I, Insulin-Like Growth Factor I - analysis, Janus Kinase 2 - analysis, Janus Kinase 2 - genetics, lactation, Lactation - genetics, Lactation - physiology, liver, Liver - chemistry, Liver - metabolism, messenger RNA, Milk and cheese industries. Ice creams, milk production, Pregnancy, Pregnancy, Animal - genetics, Pregnancy, Animal - physiology, quantitative polymerase chain reaction, Quantitative Trait, Heritable, Receptors, Somatotropin - analysis, Receptors, Somatotropin - genetics, somatotropic axis, somatotropin receptors, STAT5 Transcription Factor - analysis, STAT5 Transcription Factor - genetics, Suppressor of Cytokine Signaling Proteins - analysis, Suppressor of Cytokine Signaling Proteins - genetics, Terrestrial animal productions, transcription factors, tyrosine, Vertebrates