Sie befinden Sich nicht im Netzwerk der Universität Paderborn. Der Zugriff auf elektronische Ressourcen ist gegebenenfalls nur via VPN oder Shibboleth (DFN-AAI) möglich. mehr Informationen...
Analysis of Folate Form Distribution in Spanish Beers Using Combined Affinity and Ion-Pair Chromatography
Ist Teil von
Journal of the Institute of Brewing, 2011, Vol.117 (2), p.188-194
Ort / Verlag
Oxford, UK: Blackwell Publishing Ltd
Erscheinungsjahr
2011
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Folates and derivatives usually occur as polyglutamates, in different oxidized forms and substitutions in nature. The multiplicity of forms and the generally low content in food makes quantitative analysis of folate a difficult task. A method that uses affinity chromatography followed by ion-pair high performance liquid chromatography was adapted to the analysis of folate distribution in Spanish beers, with simultaneous information on the pteridine ring structure and the glutamate residues. On average, total folate concentration (as sum of individual vitamers expressed in folic acid) in the analyzed beers was 2.0 micrograms/100 mL. For all the beers, folates were present as monoglutamates and the analysis showed a total absence of polyglutamyl folates. The distribution of the monoglutamyl folate derivatives analyzed in Spanish beers was: 10-formyltetrahydrofolate (41.7% of total folate), tetrahydrofolate (26.5%), 5-methyltetrahydrofolate (17.2%) and folic acid (14.6%). This study provides new information regarding the distribution of naturally occurring folates in beer and contributes to the assessment of folate bioavailability in this beverage.