Details

Autor(en) / Beteiligte

• Ribeiro, C
• Panadero, J A
• Sencadas, V
• Lanceros-Méndez, S
• Tamaño, M N
• Moratal, D
• Salmerón-Sánchez, M
• Gómez Ribelles, J L

Titel

Fibronectin adsorption and cell response on electroactive poly(vinylidene fluoride) films

Ist Teil von

• Biomedical materials (Bristol), 2012-06, Vol.7 (3), p.035004-035004

Ort / Verlag

England: IOP Publishing

Erscheinungsjahr

2012

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Due to the large potential of electroactive materials in novel tissue engineering strategies, the aim of this work is to determine if the crystalline phase and or the surface electrical charge of electroactive poly(vinylidene fluoride), PVDF, have influence on the biological response in monolayer cell culture. Non-polar α-PVDF and electroactive β-PVDF were prepared. The β-PVDF films were poled by corona discharge to show negative or positive electrical surface charge density. It has been concluded that hydrophilicity of the PVDF substrates depends significantly on crystalline phase and polarity. Furthermore, by means of atomic force microscopy and an enzyme-linked immunosorbent assay test, it has been shown that positive or negative poling strongly influences the behavior of β-PVDF supports with respect to fibronectin (FN) adsorption, varying the exhibition of adhesion ligands of adsorbed FN. Culture of MC3T3-E1 pre-osteoeblasts proved that cell proliferation depends on surface polarity as well. These results open the viability of cell culture stimulation by mechanical deformation of a piezoelectric substrate that results in varying electrical charge densities on the substrate surface.