Cell host & microbe, 2012-04, Vol.11 (4), p.387-396
2012
Details
- Autor(en) / Beteiligte
- Titel
- Lactocepin Secreted By Lactobacillus Exerts Anti-Inflammatory Effects By Selectively Degrading Proinflammatory Chemokines
- Ist Teil von
- Cell host & microbe, 2012-04, Vol.11 (4), p.387-396
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The intestinal microbiota has been linked to inflammatory bowel diseases (IBD), and oral treatment with specific bacteria can ameliorate IBD. One bacterial mixture, VSL#3, containing Lactobacillus, Bifidobacterium, and Streptococcus, was clinically shown to reduce inflammation in IBD patients and normalize intestinal levels of IP-10, a lymphocyte-recruiting chemokine, in a murine colitis model. We identified Lactobacillus paracasei prtP-encoded lactocepin as a protease that selectively degrades secreted, cell-associated, and tissue-distributed IP-10, resulting in significantly reduced lymphocyte recruitment after intraperitoneal injection in an ileitis model. A human Lactobacillus casei isolate was also found to encode lactocepin and degrade IP-10. L. casei feeding studies in a murine colitis model (T cell transferred Rag2−/− mice) revealed that a prtP-disruption mutant was significantly less potent in reducing IP-10 levels, T cell infiltration and inflammation in cecal tissue compared to the isogenic wild-type strain. Thus, lactocepin-based therapies may be effective treatments for chemokine-mediated diseases like IBD. [Display omitted] ► PrtP-encoded lactocepin degrades proinflammatory chemokines, notably IP-10 ► Lactocepin reduces lymphocyte infiltration in an ileitis model ► Lactocepin lowers IP-10 levels and cecal inflammation in a colitis model ► Lactocepin-based therapy may be a treatment option for chemokine-mediated diseases
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1931-3128eISSN: 1934-6069DOI: 10.1016/j.chom.2012.02.006Titel-ID: cdi_proquest_miscellaneous_1009130313
- Format
- –
- Schlagworte
- Amino Acid Sequence, Animals, Chemokine CXCL10 - genetics, Chemokine CXCL10 - immunology, Chemokine CXCL10 - metabolism, Colitis - immunology, Colitis - metabolism, Colitis - microbiology, Colitis - therapy, Disease Models, Animal, Female, Humans, Lactobacillus - enzymology, Lactobacillus - immunology, Lactobacillus casei - enzymology, Lactobacillus casei - immunology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Molecular Sequence Data, Probiotics - therapeutic use, Protein Transport, Proteolysis, Serine Endopeptidases - immunology, Serine Endopeptidases - metabolism