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Web-Based Applet Is a Learning Tool that Simulates Ion-Exchange Chromatography Purification of Overexpressed Proteins from Escherichia coli Cell Lysate
Ist Teil von
Journal of chemical education, 2012-03, Vol.89 (4), p.555-556
Ort / Verlag
Easton: American Chemical Society and Division of Chemical Education, Inc
Erscheinungsjahr
2012
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
A Java Web applet is described that helps biochemistry students learn how varying ionic strength and pH can affect separation of proteins in an ion-exchange chromatography experiment. The applet simulates a common scenario: a foreign protein is overexpressed in bacteria and needs to be isolated from the other water-soluble proteins of the cell lysate. It allows visualization of proteins on the column, a protein output graph (absorbance at 280 nm), and polyacrylamide gel electrophoresis analysis of selected output fractions.