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Mitochondrial DNA copy number and hnRNP A2/B1 protein: Biomarkers for direct exposure of benzene
Environmental toxicology and chemistry, 2011-12, Vol.30 (12), p.2762-2770
Eom, Ha-Young
Kim, Hye-Ran
Kim, Hwan-Young
Han, Dong-Kyun
Baek, Hee-Jo
Lee, Jae-Hyuk
Moon, Jai Dong
Shin, Jong-Hee
Suh, Soon-Pal
Ryang, Dong-Wook
Kook, Hoon
Shin, Myung-Geun
2011
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Eom, Ha-Young
Kim, Hye-Ran
Kim, Hwan-Young
Han, Dong-Kyun
Baek, Hee-Jo
Lee, Jae-Hyuk
Moon, Jai Dong
Shin, Jong-Hee
Suh, Soon-Pal
Ryang, Dong-Wook
Kook, Hoon
Shin, Myung-Geun
Titel
Mitochondrial DNA copy number and hnRNP A2/B1 protein: Biomarkers for direct exposure of benzene
Ist Teil von
Environmental toxicology and chemistry, 2011-12, Vol.30 (12), p.2762-2770
Ort / Verlag
Hoboken, USA: John Wiley & Sons, Inc
Erscheinungsjahr
2011
Quelle
Wiley-Blackwell Full Collection
Beschreibungen/Notizen
The present study was performed to identify biomarkers for exposure of benzene in blood cells and hematopoietic tissues. Peripheral mononuclear cells, hematopoietic stem cells, and leukemia cell lines were cultured in RPMI 1640 media with the addition of 0, 1, and 10 mM of benzene. Hydrogen peroxide was measured using an enzyme immunoassay. Mitochondrial mass, membrane potential, and mitochondrial DNA (mtDNA) copy number were measured using MitoTracker Green/Red probes, and real‐time polymerase chain reaction. In addition, two‐dimensional gel electrophoresis and mass spectrometry matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) technology were performed to identify protein markers. The mitochondrial contents and membrane potentials were dramatically increased after three weeks of direct benzene exposure. The hydrogen peroxide level increased significantly after two weeks of treatment with benzene (4.4 ± 1.9 µM/mg protein) compared to the non‐benzene treatment group (1.2 ± 1.0; p = 0.001). The mtDNA copy number gradually increased after exposure to benzene. Numerous protein markers showed significant aberrant expression after exposure to benzene. Among them, the heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 was markedly decreased after exposure to benzene. Thus, increased mitochondrial mass, mtDNA copy number, and the hnRNP A2/B1 protein were biomarkers for benzene‐related toxicity and hematotoxicity. Environ. Toxicol. Chem. 2011;30:2762–2770. © 2011 SETAC
Sprache
Englisch
Identifikatoren
ISSN: 0730-7268
eISSN: 1552-8618
DOI: 10.1002/etc.675
Titel-ID: cdi_proquest_journals_904536614
Format
–
Schlagworte
Benzene
,
Benzene - toxicity
,
Biomarker
,
Biomarkers
,
Biomarkers - metabolism
,
Cell Line, Tumor
,
DNA, Mitochondrial - metabolism
,
Electrophoresis, Gel, Two-Dimensional
,
Environmental Monitoring - methods
,
Exposure
,
Hazardous Substances - toxicity
,
Hematotoxicity
,
Heterogeneous-Nuclear Ribonucleoprotein Group A-B - genetics
,
Heterogeneous-Nuclear Ribonucleoprotein Group A-B - metabolism
,
hnRNP A2/B1
,
Humans
,
Hydrocarbons
,
Hydrogen peroxide
,
Ionization
,
Leukemia
,
Mass spectrometry
,
Mitochondria
,
Mitochondrial DNA
,
Proteins
,
Real-Time Polymerase Chain Reaction
,
Stem cells
,
Toxicity
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