Proceedings of the National Academy of Sciences - PNAS, 2011-09, Vol.108 (37), p.E681
2011
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- Autor(en) / Beteiligte
- Titel
- Huntingtin-interacting protein 14 is a type 1 diabetes candidate protein regulating insulin secretion and [Beta]-cell apoptosis
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 2011-09, Vol.108 (37), p.E681
- Ort / Verlag
- Washington: National Academy of Sciences
- Erscheinungsjahr
- 2011
- Quelle
- EZB Electronic Journals Library
- Beschreibungen/Notizen
- Type 1 diabetes (T1D) is a complex disease characterized by the loss of insulin-secreting β-cells. Although the disease has a strong genetic component, and several loci are known to increase T1D susceptibility risk, only few causal genes have currently been identified. To identify disease-causing genes in T1D, we performed an in silico "phenome-interactome analysis" on a genome-wide linkage scan dataset. This method prioritizes candidates according to their physical interactions at the protein level with other proteins involved in diabetes. A total of 11 genes were predicted to be likely disease genes in T1D, including the INS gene. An unexpected top-scoring candidate gene was huntingtin-interacting protein (HIP)-14/ZDHHC17. Immunohistochemical analysis of pancreatic sections demonstrated that HIP14 is almost exclusively expressed in insulin-positive cells in islets of Langerhans. RNAi knockdown experiments established that HIP14 is an antiapoptotic protein required for β-cell survival and glucose-stimulated insulin secretion. Proinflammatory cytokines (IL-1β and IFN-γ) that mediate β-cell dysfunction in T1D down-regulated HIP14 expression in insulin-secreting INS-1 cells and in isolated rat and human islets. Overexpression of HIP14 was associated with a decrease in IL-1β-induced NF-kB activity and protection against IL-1β-mediated apoptosis. Our study demonstrates that the current network biology approach is a valid method to identify genes of importance for T1D and may therefore embody the basis for more rational and targeted therapeutic approaches. [PUBLICATION ABSTRACT]