Details

Autor(en) / Beteiligte

• Ross, Alastair B
• Vuong, Le Thuy
• Ruckle, Jon
• Synal, Hans Arno
• Schulze-König, Tim
• Wertz, Karin
• Rümbeli, Robert
• Liberman, Rosa G
• Skipper, Paul L
• Tannenbaum, Steven R
• Bourgeois, Alexandre
• Guy, Philippe A
• Enslen, Marc
• Nielsen, Inge Lise F
• Kochhar, Sunil
• Richelle, Myriam
• Fay, Laurent B
• Williamson, Gary

Titel

Lycopene bioavailability and metabolism in humans: an accelerator mass spectrometry study

Ist Teil von

• The American journal of clinical nutrition, 2011-06, Vol.93 (6), p.1263-1273

Ort / Verlag

Bethesda, MD: Elsevier Inc

Erscheinungsjahr

2011

Quelle

Oxford Journals 2020 Medicine

Beschreibungen/Notizen

• Background: To our knowledge, there is no direct information on lycopene metabolism in humans.Objective: The objective of this study was to quantify the long-term human bioavailability of lycopene in plasma and skin after a single dose of 14C-lycopene and to profile the metabolites formed.Design: We preselected 2 male subjects as lycopene absorbers and gave them an oral dose of 10 mg synthetic lycopene combined with ≈6 μg [6,6′,7,7′-14C]lycopene (≈30,000 Bq; 92% trans lycopene). The appearance of 14C in plasma, plasma triacylglycerol–rich lipoprotein (TRL) fraction, urine, expired breath carbon dioxide, and skin biopsies was measured over 42 d. The 14C in lycopene-isomer fractions from plasma and TRL fraction was measured to assess the isomerization of lycopene in vivo.Results: We quantified 14C from 14C-lycopene in plasma, the plasma TRL fraction, expired carbon dioxide, urine, and skin. The time to maximum concentration (tmax) of total 14C-lycopene in plasma was 6 h, and the elimination half-life (t1/2) was 5 d, which were different from the tmax and t1/2 of unlabeled lycopene (0.5 and 48 d, respectively). 14C-Lycopene was extensively isomerized after dosing as a 92% all-trans isomer at dosing but changed to 50% trans, 38% 5 cis, 1% 9 cis, and 11% other cis isomers after 24 h. A similar pattern of isomerization was seen in plasma TRL fractions.Conclusions: Lycopene was extensively isomerized after dosing and rapidly metabolized into polar metabolites excreted into urine with the rapid peak of 14CO2 after dosing, which implies that β-oxidation was involved in the lycopene metabolism. Lycopene or its metabolites were detected in skin for up to 42 d.