Details

Autor(en) / Beteiligte

• Haanstra, J. P. W.
• Wye, C.
• Verbakel, H.
• Meijer-Dekens, F.
• van den Berg, P.
• Odinot, P.
• van Heusden, A. W.
• Tanksley, S.
• Lindhout, P.
• Peleman, J.

Titel

An integrated high-density RFLP-AFLP map of tomato based on two Lycopersicon esculentum×L. pennellii F2 populations

Ist Teil von

• Theoretical and applied genetics, 1999-07, Vol.99 (1-2), p.254-271

Ort / Verlag

Heidelberg: Springer Nature B.V

Erscheinungsjahr

1999

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Two independent F^sub 2^ populations of Lycopersicon esculentum×L. pennellii which have previously been investigated in RFLP mapping studies were used for construction of a highly saturated integrated AFLP map. This map spanned 1482cM and contained 67 RFLP markers, 1078 AFLP markers obtained with 22 EcoRI+MseI primer combinations and 97 AFLP markers obtained with five PstI+MseI primer combinations, 231 AFLP markers being common to both populations. The EcoRI+MseI AFLP markers were not evenly distributed over the chromosomes. Around the centromeric region, 848 EcoRI+ MseI AFLP markers were clustered and covered a genetic distance of 199cM, corresponding to one EcoRI+ MseI AFLP marker per 0.23cM; on the distal parts 1283cM were covered by 230 EcoRI+MseI AFLP markers, corresponding to one marker per 5.6cM. The PstI/MseI AFLP markers showed a more even distribution with 16 PstI/MseI AFLP markers covering a genetic distance of 199cM around the centromeric regions and 81 PstI/MseI AFLP markers covering a genetic distance of 1283cM on the more distal parts, corresponding to one marker per 12 and 16cM respectively. In both populations a large number of loci showed a significant skewed segregation, but only chromosome 10 loci showed skewness that was similar for both populations. This ultra-dense molecular-marker map provides good perspectives for genetic and breeding purposes and map-based cloning.[PUBLICATION ABSTRACT]