Details

Autor(en) / Beteiligte

• Mitsuhashi, Shinya
• Shima, Hiroshi
• Tanuma, Nobuhiro
• Sasa, Sumie
• Onoe, Kazunori
• Ubukata, Makoto
• Kikuchi, Kunimi

Titel

Protein phosphatase type 2A, PP2A, is involved in degradation of gp130

Ist Teil von

• Molecular and cellular biochemistry, 2005-01, Vol.269 (1-2), p.183-187

Ort / Verlag

Netherlands: Springer Nature B.V

Erscheinungsjahr

2005

Quelle

MEDLINE

Beschreibungen/Notizen

• The interleukin-6 (IL-6) stimulates growth in cells such as multiple myeloma and B-cell plasmacytomas/hybridomas, while it inhibits growth in several myeloid leukemia cells. The IL-6 receptor has subunit called gp130. It was reported that Ser-782 of gp130 is phosphorylated by unidentified kinase(s) in cell extracts, and level of gp130 (S782A) transiently expressed on the cell surface of COS-7 is 6-times higher than that of the wild type. These results motivated us to analyze whether the phosphorylation of gp130 at Ser-782 is involved in its degradation or not. In this study, we demonstrated here that treatment of HepG2 cells with okadaic acid (OA), a potent inhibitor for PP2A, promotes phosphorylation of gp 130 at Ser-782 and degradation of gp 130. MG115, a proteasome inhibitor, suppressed this degradation. These effects of OA could not be replaced with tautomycetin (TC), an inhibitor for PP1. Purified PP2A dephosphorylated phospho-Ser-782 of gp130 in vitro. IL-6-induced activation of Stat3 was suppressed by preincubation of the cells with OA, suggesting that the IL-6 signaling pathway was blocked by OA through degradation of gp 130. Taken together, present results strongly suggest that degradation of gp 130 is regulated through a phosphorylation-dephosphorylation mechanism in which PP2A is crucially involved and that gp 130 is a potential therapeutic target in cancers.