Details

Autor(en) / Beteiligte

• Wang, Q. Y.
• Guo, P.
• Duan, L. L.
• Shen, Z. H.
• Chen, H. L.

Titel

1,3-Fucosyltransferase-VII stimulates the growth of hepatocarcinoma cells via the cyclin-dependent kinase inhibitor p27Kip1

Ist Teil von

• Cellular and molecular life sciences : CMLS, 2005-01, Vol.62 (2), p.171-178

Ort / Verlag

Basel: Springer Nature B.V

Erscheinungsjahr

2005

Quelle

SpringerNature Journals

Beschreibungen/Notizen

• After the transfection of α-1,3-fucosyltransferase (FucT)-VII cDNA into H7721 human hepatocarcinoma cells, the protein expression of some cyclins, cyclin-dependent kinases (CDKs) and cyclin-dependent kinase inhibitors (CDIs) p16^sup INK4^ and p21^sup waf1/Cip1^ were unchanged. However, CDI p27^sup Kip1^ protein, both the total amount and the amount that bound to CDK2, but not its mRNA, was significantly reduced. The de-inhibited CDK2 stimulated the phosphorylation of retinoblastoma (Rb) protein and facilitated the G1/S transition and growth rate of the cells. The decrease of p27^sup Kip1^ protein, the increase of CDK2 activity and Rb phosphorylation, as well as the cell growth and percentage of S phase cells were correlated to the increased amount of cell surface sialyl Lewis X (SLe^sup x^) antigen in cells with different α-1,3-FucT-VII expression. The reduction in p27^sup Kip1^ and the difference in its expression among different transfected cells were blocked by the SLe^sup x^ antibody KM93 in a dose-dependent manner, indicating that p27^sup Kip1^ expression was influenced by α-1,3-FucT-VII and its product SLe^sup x^. The MEK/MAPK signaling pathway was more important than the PI-3K pathway in the regulation of p27^sup Kip1^ expression.[PUBLICATION ABSTRACT]