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Gordonibacillus kamchatkensis gen. nov., sp. nov. from the Frozen Volcanic Ash
Ist Teil von
Microbiology (New York), 2024-06, Vol.93 (3), p.269-281
Ort / Verlag
Moscow: Pleiades Publishing
Erscheinungsjahr
2024
Link zum Volltext
Quelle
2022 ECC(Springer)
Beschreibungen/Notizen
An aerobic, rod-shaped, non-motile, endospore-forming bacterium, strain V-9
T
, was found in the frozen volcanic ash, Kamchatka peninsula, Russia. Phylogenies based on 16S rRNA gene and genome sequences demonstrated that strain V-9
T
was affiliated with the family
Paenibacillaceae
and clustered outside the clade that includes the type species of this genus,
Paenibacillus polymyxa,
and closely related species. V-9
T
showed the highest 16S rRNA gene sequences similarity to
Paenibacillus chartarius
CCUG 55240
T
(96.7%). The similarity to type strains of other
Paenibacillus
species were below 94.7%, with 91.8% determined to
Paenibacillus polymyxa
IAM 13419
T
.
The AAI and POCP values between V-9
T
and type species of the genera of
Paenibacillaceae
with available genomes, including
Paenibacillus polymyxa,
were below the thresholds for defining novel genera. The cell wall of new strain contained a lysine-based peptidoglycan and two teichoic acids, unsubstituted 1,3-poly(glycerol phosphate) and 1,3-poly(glycerol phosphate) substituted at the C(2) of glycerol by glucose. Acid hydrolysates of the cell wall included glucose, galactose and glycerol. Strain V-9
T
contained menaquinone MK-7 as the sole isoprenoid quinone, anteiso-C
15:0
, anteiso-C
17:0
, iso- C
15:0
and iso-C
16:0
a as the dominant fatty acids, and phosphatidylglycerol, diphosphatidylglycerol, acylphosphatidylglycerol, and phosphatidylinositol as the major polar lipids. The DNA G+C content calculated from the whole genome sequence was 55 mol %. Based on the data obtained, a new genus and species,
Gordonibacillus kamchatkensis
gen. nov., sp. nov., is proposed with the type strain V-9
T
(=VKM B-2647
T
= DSM 26726
T
).