Details
- Autor(en) / Beteiligte
- Titel
- The development, characterization and wound healing in the keratin-14-promoted transforming growth factor-beta 1 transgenic mouse
- Ort / Verlag
- ProQuest Dissertations & Theses
- Erscheinungsjahr
- 2000
- Link zum Volltext
- Quelle
- ProQuest Dissertations & Theses A&I
- Beschreibungen/Notizen
- Transforming growth factor-beta 1 (TGF-β1) stimulates extracellular matrix synthesis at the same time inhibiting matrix degradation (Gleizes et al, 1997; Zhang et al, 1995). The study of a genetically manipulated animal, which produces excessive TGF-β in the skin, would be useful to evaluate its role in the epidermis and may help to further understand the development of fibrosis. We developed a transgenic mouse which specifically targeted human TGF-β1 to the skin using a keratin 14 promoter and was subsequently bred into homozygous and heterozygous lines. The transgene was shown to be preferentially expressed in the skin without any detectable systemic effects. The only phenotypic difference noted was signs of ‘scruffiness’ in only the homozygous lines of mice. Wound healing in these animals showed increased levels of TGF-β1 protein in the transgenic animals which would explain the delayed re-epithelialization observed. The less than dramatic effects in the dermis post-wounding are attributed to the lack of sufficient activation of the latent TGF-β1 in these animals. In conclusion, we have developed a transgenic mouse that will be useful in future studies and have shown that excessive TGF-β1 from keratinocytes can impede wound healing.