Details

Autor(en) / Beteiligte

• Olivieri, Wilma

Titel

Effects of dietary galactose on porcine feto-placental glucose metabolism, neonatal energy storage, and survival

Ort / Verlag

ProQuest Dissertations & Theses

Erscheinungsjahr

1989

Quelle

ProQuest Dissertations & Theses A&I

Beschreibungen/Notizen

• Thirty-two crossbred gilts were divided randomly into four groups of eight animals each and fed 1.82 kg/animal of diets containing galactose and glucose in percentages of 0:24, 6:18, 12:12, or 24:0. These diets were fed from day 98 to 112 of gestation. Two piglets were collected at parturition for analysis of plasma glucose and galactose; liver and semitendinosus muscle glycogen; and total body lipid composition. Two additional piglets were fasted 24 h at 27$\sp\circ$C for analysis of the ability to maintain blood glucose and galactose during a 24 h fast. Maternal plasma galactose concentrations increased with increasing dietary galactose (P $<$.05), while plasma glucose decreased with decreasing dietary glucose. These changes in maternal blood parameters had no apparent effects on plasma glucose or galactose, deposition of tissue glycogen, or body lipid content of neonatal pigs. Feeding a high level of galactose in the maternal diet resulted in an increase in plasma glucose levels of fasting and neonatal pigs, suggesting a difference in the turnover of tissue energy reserves (P $<$.05). In a second experiment, pregnant gilts were fed diets containing either 24% glucose (control) or 24% galactose from day 98 to 110 of gestation when caesarean section was performed. Slices of subcutaneous adipose tissue (SAT) and placenta were incubated for 2 h in Krebs-Ringer bicarbonate buffer containing 2% bovine serum albumin, 10 mM glucose $\pm$10 mM galactose and 1.0 $\mu$Ci (U-$\sp{14}$C) glucose. No effects of maternal diet were evident upon glycogen content or enzyme activity of fetal semitendinosus and liver. Maternal dietary galactose had no direct effects upon placental glucose metabolism. However, galactose supplementation of the incubation medium caused reductions in glucose oxidation (15%) and total lipid synthesis (24%) by the maternal placenta. Maternal dietary galactose caused an increase in total lipid (50%) and fatty acid synthesis (200%) from glucose in fetal SAT; direct supplementation of galactose to the incubation medium had no effect. These data suggest maternal dietary galactose may spare glucose from utilization by maternal tissue for use by the fetus for lipogenesis but galactose may not directly affect fetal energy accretion.