Details

Autor(en) / Beteiligte

• Wu, Fan
• Li, Shangyu
• Chen, Xun
• Yue, Shuhua
• Hong, Weili
• Wang, Pu

Titel

High‐Sensitive and Background‐Free Coherent Anti‐Stokes Raman Scattering Microscopy Using Delay Modulation

Ist Teil von

• Laser & photonics reviews, 2024-04, Vol.18 (4), p.n/a

Ort / Verlag

Weinheim: Wiley Subscription Services, Inc

Erscheinungsjahr

2024

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Coherent anti‐Stokes Raman scattering (CARS) microscopy has demonstrated a powerful platform for label‐free, non‐invasive, and chemically specific imaging of biological samples. However, the non‐resonant background limits its ability to detect weak Raman signals. Here, an approach to eliminate the non‐resonant background in CARS using delay modulation (DM) is presented, which is enabled by an acousto‐optic modulator and spectral focusing. The results demonstrate that DM‐CARS reduces the non‐resonant background by 10 times and improves detection sensitivity by 100‐fold over normal CARS. It also shows that DM‐CARS has potential applications in tracking heavy water metabolism in bacteria for antimicrobial susceptibility testing and imaging liver tumor tissues. Furthermore, a handheld DM‐CARS device has also demonstrated for in vivo imaging, which can be beneficial in various applications. This delay modulation coherent anti‐Stokes Raman scattering (DM‐CARS) microscopy effectively reduces the non‐resonant background of coherent anti‐Stokes Raman scattering (CARS) by 10 times and improves detection sensitivity by 100‐fold over normal CARS, which is enabled by an acousto‐optic modulator and spectral focusing. A handheld DM‐CARS device is also demonstrated.