Details

Autor(en) / Beteiligte

• SENATORE, TUNATO FRED

Titel

DEVELOPMENT OF A BIOCOMPATIBLE VASCULAR PROSTHESIS

Ort / Verlag

ProQuest Dissertations & Theses

Erscheinungsjahr

1983

Quelle

ProQuest Dissertations & Theses A&I

Beschreibungen/Notizen

• The use of chemical engineering analysis is applied towards medical research in an attempt to develop a biocompatible vascular prosthesis. The synthesis of the fibrocollagenous tube is accomplished by implanting into the parniculus carnosis of healthy dogs a silicone rubber rod which has previously been surrounded by a 70 veneer dacron mesh. In twelve weeks collagenous deposits are interwoven with the dacron. The fibrocollagenous tube is excised and the silicone rubber rod is removed leaving the collagen-dacron complex. The collagenous tube is usually 4 mm in internal diameter. The enzyme urokinase is immobilized on the inner surface of the collagenous tube in order to render the prosthesis thrombolytic. Urokinase activity is evaluated in vitro by esterolysis using a differential recirculation reactor with low conversion per pass thereby approximating a batch system. The differential recirculation system allows mass transfer and intrinsic kinetic analysis which will elucidate the overall kinetic mechanism of bound urokinase activity. Results indicate that mass transfer is comparably insignificant to an intrinsic association between the enzyme and a dispersed collagen solution. Stability criterion, optimum crosslink density, and storage conditions are also evaluated by the differential recirculation reactor. Urokinase esterolytic activity remains stable for over one year and is unaffected by exposure to blood. A 1 percent glutaraldehyde crosslink density is optimum.