Details

Autor(en) / Beteiligte

• Khammo, N

Titel

Development of in vitro models for the assessment of stimulatory/inhibitory factors in burn patient serum

Ort / Verlag

ProQuest Dissertations & Theses

Erscheinungsjahr

1996

Quelle

ProQuest Dissertations & Theses A&I

Beschreibungen/Notizen

• The primary aim of this study was to demonstrate the in vitro effects of burn patient serum on healthy human skin cells involved in wound healing. Serum was obtained from the blood of five burn patients during the early stages post-burn and at regular intervals until patient discharge or death. The patients' burn injuries ranged between <10%-65% total body surface area.The effect of burn patient serum on fibroblast and keratinocyte cell MTT-conversion was determined. An inhibitory or stimulatory effect was observed with serum samples taken during the early post-burn period. It was interesting to note that a stimulatory effect was observed with the one patient who died whereas an inhibitory effect was observed with serum from the surviving patients.The effect of burn patient serum on cell migration was also investigated. Serum taken during the early post-burn period from patients with large surface area burns involving full-thickness skin loss had a lower fibroblast chemokinetic activity than normal human serum.This was the first study to evaluate the effect of burn patient serum on skin cells and has succeeded in demonstrating inhibitory and stimulatory effects of burn patient serum on viable fibroblasts and keratinocytes. The rationale of the study was to intensively investigate a small patient population in order to include as many serum samples as possible, and to utilise each of the samples in many different assays of cell function and morphology. Inevitably, this resulted in limited conclusions with respect to patient-related characteristics such as burn size, type of burn and clinical outcome. Nevertheless, trends were evident and these provide encouragement for further studies involving a larger number of patients.