Details

Autor(en) / Beteiligte

• Schmidt, P. P.
• Kappl, R.
• Hüttermann, J.

Titel

On the mode of hexacoordinated NO-binding to myo- and hemoglobin: Variable-temperature EPR studies at multiple microwave frequencies

Ist Teil von

• Applied magnetic resonance, 2001-12, Vol.21 (3-4), p.423-440

Ort / Verlag

Heidelberg: Springer Nature B.V

Erscheinungsjahr

2001

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Electron paramagnetic resonance (EPR) spectroscopy is performed on NO⋅-ligated hemo- and myoglobin at temperatures between 5 and 300 K. Apart from the standard X-band (9.5 GHz), data are taken at K-(24 GHz), Q-(34 GHz), W-(94 GHz) and Y-band (285 GHz) frequencies, respectively. The spectra are delineated into contributions of two EPR signatures differing ing-tensor symmetry, an axial and a rhombic one. The state contributions are found to differ with temperature and the states show temperature-dependentg-factor variations. In MbNO the axial species (denoted state II) is the only species observed at 300 K with X-band. Analysis of its development with decreasing temperature shows a partial transformation into a rhombic state (I). For this, at an intermediate temperature range (240–100 K), irregular line shapes are apparent, indicating disordered geometries of the Fe-NO-heme group. Regular rhombic lineshapes develop increasingly at temperatures below about 70 K. At 5–10 K, the rhombic state I dominates the EPR spectra but state II is still present. A similar development of spectra is observed in HbNO, for which, however, a rhombic contribution is already present at 300 K. Subunit-associated variations in state contributions and EPR appearance are not resolved at any frequency. Also, lineshape irregularities with decreasing temperature are not manifest in HbNO. These findings are used to present a structure-based picture of states I and II and their transformations and to discuss the origin of the line shape irregularities in MbNO.