Details

Autor(en) / Beteiligte

• Frattini, Laís M.
• Oliveira, Arthur A.
• Kotsubo, Jhoão S.
• Gorne, Íris B.
• Silva, Isabel C.
• Freitas, Jober C. E.
• Resende, Cristiano F.
• Mezzonato-Pires, Ana C.
• Matos, Elyabe M.
• Lopes, Juliana M. L.
• Viccini, Lyderson F.
• Grazul, Richard M.
• Peixoto, Paulo H. P.

Titel

Enhancing the taxonomic delimitation of Lippia origanoides Kunth (Verbenaceae) by analyzing volatile terpenes and molecular markers in micropropagated accessions

Ist Teil von

• Plant cell, tissue and organ culture, 2024, Vol.156 (1), p.17, Article 17

Ort / Verlag

Dordrecht: Springer Netherlands

Erscheinungsjahr

2024

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• This study aimed to contribute to the accurate taxonomic delimitation of Lippia origanoides Kunth (Verbenaceae), an important native Brazilian herb with diverse medicinal properties, culinary uses and wide phenotypic plasticity. For this purpose, the large-scale in vitro propagation of accessions of L. origanoides , initially identified as L. microphylla (two accessions), L. sidoides and L. salviaefolia , was established. In addition, the composition of the three major volatile organic terpenes in these accessions was compared. The micropropagation protocols revealed differences in the proliferative responses of the different accessions; in contrast, there was a predominance of the terpenes thymol, thymol methyl ether and thujopsene in the volatile organic fraction, regardless of the Lippia accession evaluated. The correlation analyses grouped the two L. microphylla accessions in poles opposite to the other two accessions ( L. sidoides and L. salviaefolia ), but multivariate principal component analysis (PCA) considering the major terpenes present in the organic fraction grouped the four accessions near the center of the PCA, indicating similarity among them. The results of this study demonstrated that L. origanoides can be efficiently propagated in vitro. Furthermore, based on the results of the analysis of the composition of major terpenes and molecular markers, the main contribution of the present study was to corroborate the hypothesis that the dissimilarity among the evaluated Lippia accessions is insufficient for them to constitute distinct species and that they are therefore synonymous with L. origanoides . Key Message L. origanoides plants can be efficiently micropropagated, and molecular markers and the proportion of major terpenes present in its essential oil can be used in the taxonomic delimitation of the species.