Details

Autor(en) / Beteiligte

• Venkatesan, C
• Koteeswaran, M
• S Senthil Nathan
• Umamageshwaran, V
• Jagan, E
• Balasubramaniyan, S
• Balachandar, V
• S Praveen Kumar
• Maheswari, R
• Thirumagal, J

Titel

Encapsulation efficiency of Chitosan Tripolyphosphate nanoparticles containing Saw scaled viper snake venom

Ist Teil von

• NeuroQuantology, 2022-01, Vol.20 (8), p.9271

Ort / Verlag

Bornova Izmir: NeuroQuantology

Erscheinungsjahr

2022

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Chitosan Tripolyphosphate (CS/TPP) nanoparticle is a biodegradable and nontoxic polysaccharide, used as a carrier for drug delivery. The morphology and particle size measurements of the nanoparticles were studied by field emission scanning electron microscopy (FE-SEM) and FTIR (Fourier Transform Infrared Spectra). This study aims to evaluate the impact of Saw Scaled Viper venom encapsulation on various factors and loading capacity, in addition to explore the physicochemical structure of nanoparticles. FTIR confirmed that tripolyphosphoric groups of TPP linked with ammonium groups of CS in the nanoparticles. Our results showed that CS can react with TPP to form stable cationic nanoparticles. Theresults showed that encapsulation efficiency of venom at a different concentration 20, 40, 60, 500 and 1000 μg/ ml were achieved for CS/TPP nanoparticles at a different concentration of 1.5, 2 and 3 mg/ml. The cytotoxicity of CS/TPP nanoparticles was evaluated by MTT (-3 (4, 5-Dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide, a tetrazole) assay.