Details

Autor(en) / Beteiligte

• Park, Yeo-Jin
• Choi, Gyu‑Sik
• Lee, Shin-Won
• Ahn, Joong-Hoon

Titel

Production of quinolone derivatives in Escherichia coli

Ist Teil von

• Applied biological chemistry, 2022-12, Vol.65 (1), p.65, Article 65

Ort / Verlag

Singapore: Springer Nature Singapore

Erscheinungsjahr

2022

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Alkyl-4-quinolones (AQs) are natural compounds synthesized by bacteria. Members of this group are known quorum-sensing molecules. Other biological functions, such as anti-bacterial, anti-algal, antifungal, and anti-malaria activities have also been reported. The synthetic pathways of AQs have been validated in Pseudomonas aeruginosa . Five genes ( pqsA–E ) are involved in the synthesis of 2-heptyl-4(1H)-quinolone (HHQ). To synthesize HHQ in a microbial system, pqsA–E genes were introduced into Escherichia coli and HHQ and 2-methyl-4(1H)-quinolone (MHQ) were synthesized. After the copy number, construct promoters, and substrate supplements were optimized, 141.3 mg/L MHQ and 242.8 mg/L HHQ were synthesized.