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Phylogeny of Pseudoperonospora causing downy mildew of Cucurbitaceae in Brazil
Ist Teil von
European journal of plant pathology, 2023-11, Vol.167 (3), p.361-370
Ort / Verlag
Dordrecht: Springer Netherlands
Erscheinungsjahr
2023
Link zum Volltext
Quelle
Alma/SFX Local Collection
Beschreibungen/Notizen
Downy mildew of cucurbits is a disease caused by the Oomycete
Pseudoperonospora cubensis
. It can cause considerable losses in cucurbit crops. Phylogenetic studies point to the existence of cryptic species of
Pseudoperonospora
. The objective of this study was to determine, the phylogeny of
Pseudoperonospora
species affecting cucurbits in different regions of Brazil. For this, leaves with symptoms of downy mildew were collected in areas of cucurbits production in the states of Pernambuco, Minas Gerais, Ceará, and the Distrito Federal. For phylogenetic analyses, DNA extraction was performed, and PCR reactions were carried out using primers DC-6 and LR-0, Cox2-F and Cox2-RC4, OomCox1-levup and OomCox1-levlo, FM79-C1 and FMPHY-10b, for amplification of the internal transcribed spacer (ITS) region, the mitochondrial cytochrome c oxidase subunit II (cox2), the mitochondrial cytochrome c oxidase subunit I (cox1) and cox2-cox1 intergenic spacer respectively. The nucleotide sequences were analyzed, edited, and aligned to perform Bayesian Inference analysis. The results of the phylogenetic analyses presented in the cox2-cox1-cox2_1-ITS concatenated tree indicate that in Brazil, the downy mildew of cucurbits is caused by phylogenetically distinct lineages of
P. cubensis.