Details

Autor(en) / Beteiligte

• Temnov, A. A.
• Sklifas, A. N.
• Zhalimov, V. K.
• Sharapov, M. G.
• Fadeev, R. S.
• Kobyakova, M. I.
• Kukushkin, N. I.
• Rogov, K. A.

Titel

Paracrine Effects of Stem Cell Conditioned Medium on Reactive Oxygen Species Production in Blood Neutrophils in Acetaminophen-Induced Liver Failure

Ist Teil von

• Biophysics (Oxford), 2023-02, Vol.68 (1), p.70-78

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• A mesenchymal stem cell (MSC)-derived conditioned medium (CM) was tested for effects on the severity of the systemic inflammatory response induced by acetaminophen (APAP), including its long-term influence on hepatic tissue. A CM fraction of <30 kDa showed a maximum protective effect. Proteins of the fraction reduced the systemic inflammatory response and liver tissue fibrosis long after the toxin administration. Liver tissues from experimental animals were examined, and reduction of granulocytic infiltration was found to correlate with the degree of hepatic parenchymal necrosis, which was confirmed by the cytolysis enzyme level. The <30-kDa fraction increased the spontaneous and decreased the phorbol 12-myristate 13-acetate (PMA)-induced intracellular production of reactive oxygen species (ROS). Expression of the macrophage migration inhibitory factor (MIF) and thioredoxin significantly increased as compared to controls during increased spontaneous ROS production in neutrophils.