Details

Autor(en) / Beteiligte

• Fletcher, Ashley A
• Kelly, Matthew S
• Eckhoff, Austin M
• Allen, Peter J
• Xu, Fangxi
• Saxena, Deepak
• Pushalkar, Smruti
• Miller, George

Titel

Revisiting the intrinsic mycobiome in pancreatic cancer/Reply

Ist Teil von

• Nature (London), 2023-08, Vol.620 (7972), p.E1-7

Ort / Verlag

London: Nature Publishing Group

Erscheinungsjahr

2023

Beschreibungen/Notizen

• Focusing only on the analysis of these human samples, we retrieved the raw sequencing data that were publicly available through the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA) to confirm the findings from the paper and to compare the gut and pancreatic mycobiomes of the healthy participants and patients with PDAC. There were no significant differences in mycobiome composition or diversity by patient group (Fig. 1c,d). [...]although Malassezia was among the most abundant genera in these samples, this genus was inconsistently present in both healthy and PDAC pancreatic tissues (Fig. 1e). Using a DADA2 pipeline similar to ours, they also did not identify a significant fungal presence in pancreatic tissues, with only 16 reads assigned to Malasezzia spp., all of which were found in a single PDAC sample (Extended Data Fig. 2a-d). [...]given the similar results from multiple approaches to the analysis of these sequencing data, we believe that the fungal sequencing reads generated from the human pancreatic tissues in this study are insufficient for analyses of mycobiome diversity and composition and do not support the conclusion that there is an increased presence of Malasezzia in tissue from human PDAC tumours. [...]although Aykut et al. showed fungal dysbiosis in the pancreatic tissues and guts of wild-type and PDAC mice, our analyses did not identify similar differences within human pancreatic tissues or faecal samples. [...]we believe that there is currently insufficient evidence to support the hypothesis that the pancreatic or gut mycobiome promotes pancreatic oncogenesis in humans.