Details

Autor(en) / Beteiligte

• da Costa Siqueira, Juliana Tatiara
• Reis, Aryane Campos
• Lopes, Juliana Mainenti Leal
• Ladeira, Luiz Orlando
• Viccini, Lyderson Facio
• de Mello Brandão, Humberto
• Munk, Michele
• de Sousa, Saulo Marçal

Titel

Chromosomal aberrations and changes in the methylation patterns of Lactuca sativa L. (Asteraceae) exposed to carbon nanotubes

Ist Teil von

• Biológia, 2023-08, Vol.78 (8), p.1991-2002

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2023

Quelle

SpringerLink

Beschreibungen/Notizen

• Carbon nanotubes (CNTs) are one of the most important nanomaterials, with applications in several areas. The exponential increase in the production and use of CNTs, however, increases the likelihood of their release into the environment and potential impacts on environmental systems. We evaluated the cytogenotoxic potential of carboxylated multi-walled carbon nanotubes (MWCNT-COOH) as well as its influence in the methylation patterns of Lactuca sativa meristematic cells. Root elongation was observed at concentrations of 1.0; 10.0; 50.0 and 100.0 μg/mL, and decreased seed germination at the highest concentration evaluated (100.0 μg/mL). Cytogenetic analysis revealed that MWCNT-COOH induced increases in the chromosomal aberration index and micronucleus frequency. Accumulations of cells in the sub G 1 phase were also observed at low concentrations (0.1 μg / mL), which may be related to increased programmed cell death. Cytosine methylation profile analysis revealed cytosine hypermethylation at MWCNT-COOH concentrations of 1.0 and 10.0 μg / mL and cytosine hypomethylation at MWCNT-COOH concentrations of 50.0 and 100.0 μg / mL as compared to controls. We conclude that, in addition to cytogenotoxic effects, MWCNT-COOH alters cytosine methylation profiles and promotes root elongation.