Details

Autor(en) / Beteiligte

• Adams, Alexandra C.
• Kufcsak, Andras
• Ehrlich, Katjana
• Dhaliwal, Kevin
• Seth, Sohan

Titel

Simultaneous Spectral Temporal Modelling for a Time-Resolved Fluorescence Emission Spectrum

Ist Teil von

• IEEE transactions on biomedical engineering, 2023-08, Vol.70 (8), p.1-15

Ort / Verlag

United States: IEEE

Erscheinungsjahr

2023

Quelle

IEEE Xplore

Beschreibungen/Notizen

• Innovations in complementary metal-oxide semiconductor (CMOS) single-photon avalanche diode (SPAD) technology has featured in the development of next-generation instruments for point-based time-resolved fluorescence spectroscopy (TRFS). These instruments provide hundreds of spectral channels, allowing the collection of fluorescence intensity and fluorescence lifetime information over a broad spectral range at a high spectral and temporal resolution. We present Multichannel Fluorescence Lifetime Estimation, MuFLE, an efficient computational approach to exploit the unique multi-channel spectroscopy data with an emphasis on simultaneous estimation of the emission spectra, and the respective spectral fluorescence lifetimes. In addition, we show that this approach can estimate the individual spectral characteristics of fluorophores from a mixed sample.