Details

Autor(en) / Beteiligte

• St-Denis, Thomas

Titel

Characterization of Nanopores and Resensing of DNA Molecules Using a Logic-Driven Apparatus

Ort / Verlag

ProQuest Dissertations & Theses

Erscheinungsjahr

2022

Link zum Volltext

• ProQuest

Quelle

ProQuest Dissertations & Theses A&I

Beschreibungen/Notizen

• Solid-state nanopores are small holes on the nanometer scale that are widely used in biology, medicine and genomics to analyze various molecules. In particular, with a good knowledge of the characteristics of the specific nanopore used, like size, geometry and symmetry, one can determine basic features of molecules passing through the pores such as length, diameter and conformation. This project aims to better understand those characteristics by using a custom-built conditioning system capable of growing pores and rectifying their geometry while also tracking their conductance and size. Additionally, an FPGA system was made to control the direction of the flow of molecules to scan individual molecules several times (known as resensing). Not only does this system provide improvements to sensing accuracy from repeated sampling, but detailed information on transport mechanisms of molecules, such as knot-formation and folding probabilities, can be extracted.