Details

Autor(en) / Beteiligte

• Ramírez-Mosqueda, Marco A.
• de la Cruz Díaz-Sánchez, María
• Gómez-Merino, Fernando C.
• Hernández-Rosas, Francisco
• Rodríguez-Lagunes, Daniel A.
• Bello-Bello, Jericó J.

Titel

Nodal Segment Size and Encapsulation Matrix Affect Sugarcane Bud Emergence from Synthetic Seeds

Ist Teil von

• Sugar tech : an international journal of sugar crops & related industries, 2023-08, Vol.25 (4), p.827-833

Ort / Verlag

New Delhi: Springer India

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Artificial or synthetic seed consists of a coating that protects plant tissues, being an alternative for commercial propagation of sugarcane. The objective of this study was to develop a system for synthetic seed production of sugarcane. A first experiment determined the optimal nodal segments size (1.0, 2.5, and 5.0 cm long) in bud emergence; a second experiment evaluated different concentrations of sodium alginate (Na-alginate: 1%, 2% and 3% w/v) and calcium chloride (CaCl 2 : 50, 100 and 200 mM) to determine bead formation and breaking ability. At 30 days of culture were evaluated the percentage of emergence, shoot length, number of leaves and number of roots. In addition, we determined the cutting force associated with the different combinations of the encapsulation matrix. The results showed a higher percentage of emergence and development by using nodal segment measuring 2.5–5.0 cm long. The combination of 1% Na-alginate + 50 or 100 mM CaCl 2 allowed an efficient encapsulation for the formation of synthetic seed, without affecting the percentage of emergence and development variables evaluated. We described a system for the production of synthetic seeds of sugarcane, a biotechnological strategy that may be an alternative for the production of basic seed and the modernization of sugarcane cultivation.