Details

Autor(en) / Beteiligte

• Zhao, Yan
• Wang, Yan
• Zhang, Chengshuo
• Zhou, Haotian
• Song, Lingyuan
• Tu, HanQing
• Zhao, Jinliang

Titel

Variation in pH, HCO3−, carbonic anhydrases, and HCO3− transporters in Nile tilapia during carbonate alkalinity stress

Ist Teil von

• Hydrobiologia, 2023-06, Vol.850 (10-11), p.2447-2459

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2023

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Metabolite changes in Nile tilapia in response to carbonate alkalinity stress were investigated by transferring the fish directly from freshwater into different carbonate alkaline water. Levels of plasma pH/HCO 3 − concentration, the mRNA and protein expression of two carbonic anhydrases (CAhz and CAIV), and two HCO 3 − transporters (Na + /HCO 3 − cotransporter and Cl − /HCO 3 – exchanger) in the gill, kidney, and intestine were determined using a pH meter, UV spectrophotometer, quantitative real-time PCR, and western blotting within 192 h of exposure. Plasma pH showed an “up-peak-down” variation, whereas HCO 3 − concentration decreased at first and then increased in all alkaline water groups. The overall mRNA expression was regulated in an alkalinity- and time-dependent manner. Western blot results showed that the Cl − /HCO 3 − exchanger protein was detected in all tissues examined, whereas the two carbonic anhydrases and Na + /HCO 3 − cotransporter proteins were only expressed in the gill and kidney. Therefore, the studied carbonic anhydrases and HCO 3 − transporters are involved in the HCO 3 − metabolism and transport to maintain acid–base balance in Nile tilapia under carbonate alkalinity stress.