Details

Autor(en) / Beteiligte

• Nagendra, Babu V
• Koteswara Rao Gsn
• Rajasekhar, Reddy A
• Yadav, Khushwant S
• Kishore, Babu G
• Durga, Prasad A
• Nagendra Ch

Titel

RP-HPLC Analytical Method Development And Validation For Quantification Of Alpha-Mangostin Entrapped In Solid Lipid Nanoparticles

Ist Teil von

• NeuroQuantology, 2022-01, Vol.20 (17), p.695

Ort / Verlag

Bornova Izmir: NeuroQuantology

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The present study established and validated a quantitative determination of alpha-mangostin extracted from solid lipid nanoparticles of Garcinia mangostana extract, a reverse phase fast, high-pressure liquid chromatographic technique. According to the international conference on harmonization recommendations, a quick and cost-effective approach for estimating and quantifying alpha-mangosteen was designed and verified precisely and correctly. The stationary phase was activated silica with a particle size of 3.5μm based on an X-Bridge C18 guard column (4.6*100mm), resulting in quicker and more accurate separation of eluents due to the smaller particle size of silica. APhoto diode array detector was used to detect acetonitrile and 0.1 % v/v orthophosphoric acid in 65:35 volumes at a flow rate of 1ml/min at a wavelength of 317nm. With an r2 value of 0.999, linearity was confirmed in the concentration range of 17.38-260.90 g/ml. Interday and intraday variation with a relative standard deviation of 0.048 %-0.165 % and 0.036 %-0.182 %, respectively, were used to achieve precision. The linearity results revealed that the detection and quantification limits were 0.000121 μg/ml and 0.000365 μg/ml, respectively. The elution time for α- mangosteen was 7.82 min.