Details

Autor(en) / Beteiligte

• Sung, Shih-Ying
• Lin, Yi-Wen
• Wu, Chin-Chen
• Lin, Chih-Yuan
• Hsu, Po-Shun
• Periasamy, Srinivasan
• Nagarajan, Balaji
• Hsieh, Dar-Jen
• Tsai, Yi-Ting
• Tsai, Chien-Sung
• Lin, Feng-Yen

Titel

Supercritical carbon dioxide-decellularized arteries exhibit physiologic-like vessel regeneration following xenotransplantation in rats

Ist Teil von

• Biomaterials science, 2023-03, Vol.11 (7), p.2566-258

Ort / Verlag

England: Royal Society of Chemistry

Erscheinungsjahr

2023

Quelle

MEDLINE

Beschreibungen/Notizen

• Currently, many techniques are used for decellularization of grafts, including physical, enzymatic, and chemical treatments. Indeed, decellularized xenogenic grafts provide superior outcomes than alternative synthetic conduits. However, vascular grafts produced by these methods are not perfect; their defects include defective vessel wall structures, detergent residues, and the development of aneurysms after grafting. Therefore, it is essential to develop a more appropriate process to produce decellularized vascular grafts. Supercritical carbon dioxide (ScCO 2 ) has been used in decellularization technologies in recent years. It is beneficial for the long-term preservation of tissues and regeneration of new vessels. We have previously reported that ScCO 2 -produced acellular porcine corneas show excellent biocompatibility following lamellar corneal transplantation in rabbits. In this study, we wanted to use this method to fabricate vascular grafts (ScCO 2 -decellularized rabbit femoral artery (DFA)) and analyze their efficacy, parameters regarding rejection by the recipient's (ACI/NKyo rats) immune system and biocompatibility, structural regeneration, and functionality in vivo . The results indicated that the ScCO 2 -DFA showed higher biocompatibility, enhanced chemotactic migration of endothelial progenitor cells, lower risk of vasculopathy, lower inflammatory and splenic immune responses, and better physiological-like tension responses after xenotransplantation (XTP) in ACI/NKyo rats compared with the results obtained after XTP using detergent decellularized vascular grafts (SDS-DFA). In conclusion, ScCO 2 is an excellent decellularization technique in the fabrication of biocompatible vascular grafts and has tremendous application in vascular regenerative medicine. ScCO 2 -decellularized artery graft having tremendous application in vascular regenerative medicine.It with physiological-like responses, including high potential of biocompatibility and long-term patency, lower risk of aneurysm after xenotransplantation.