Details

Autor(en) / Beteiligte

• Pan, Guangtao
• Zhang, Ping
• Chen, Aiying
• Deng, Yu
• Zhang, Zhen
• Lu, Han
• Zhu, Aoxun
• Zhou, Cong
• Wu, Yanran
• Li, Sen

Titel

Aerobic glycolysis in colon cancer is repressed by naringin via the HIF1A pathway

Ist Teil von

• Journal of Zhejiang University. B. Science, 2023-03, Vol.24 (3), p.221-231

Ort / Verlag

Hangzhou: Zhejiang University Press

Erscheinungsjahr

2023

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Metabolic reprogramming is a common phenomenon in cancer, with aerobic glycolysis being one of its important characteristics. Hypoxia-inducible factor-1α ( HIF1A ) is thought to play an important role in aerobic glycolysis. Meanwhile, naringin is a natural flavanone glycoside derived from grapefruits and many other citrus fruits. In this work, we identified glycolytic genes related to HIF1A by analyzing the colon cancer database. The analysis of extracellular acidification rate and cell function verified the regulatory effects of HIF1A overexpression on glycolysis, and the proliferation and migration of colon cancer cells. Moreover, naringin was used as an inhibitor of colon cancer cells to illustrate its effect on HIF1A function. The results showed that the HIF1A and enolase 2 ( ENO2 ) levels in colon cancer tissues were highly correlated, and their high expression indicated a poor prognosis for colon cancer patients. Mechanistically, HIF1A directly binds to the DNA promoter region and upregulates the transcription of ENO2; ectopic expression of ENO2 increased aerobic glycolysis in colon cancer cells. Most importantly, we found that the appropriate concentration of naringin inhibited the transcriptional activity of HIF1A , which in turn decreased aerobic glycolysis in colon cancer cells. Generally, naringin reduces glycolysis in colon cancer cells by reducing the transcriptional activity of HIF1A and the proliferation and invasion of colon cancer cells. This study helps to elucidate the relationship between colon cancer progression and glucose metabolism, and demonstrates the efficacy of naringin in the treatment of colon cancer.