Details

Autor(en) / Beteiligte

• Alpatieva, N. V.
• Anisimova, I. N.
• Radchenko, E. E.

Titel

Mitochondrial ND4 Gene as a Marker Trait for Studying Structure and Dynamics of Aphid (Hemiptera, Aphididae) Populations

Ist Teil von

• Entomological review, 2022-09, Vol.102 (6), p.820-828

Ort / Verlag

Moscow: Pleiades Publishing

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Next-generation sequencing (NGS) was used to study polymorphism in a fragment of the ND4 gene encoding NADH dehydrogenase subunit 4. The point substitution range was evaluated in populations of the cowpea aphid Aphis craccivora C.L. Koch, the English grain aphid Sitobion avenae Fabricius, the bird cherry-oat aphid Rhopalosiphum padi L., and the greenbug Schizaphis graminum Rondani collected in Krasnodar Territory and in Northwestern Russia. The detected mitochondrial haplotypes matched the reference sequences by 96–100%. The level of intraspecific polymorphism of the ND4 gene was 0.2–4.0% in A. craccivora , 0.2–1.9% in S. avenae , 0.9–3.3% in Rh. padi , and 0.2–0.9% in Sch. graminum . The proportion of polymorphic sites in the ND4 gene sequences among different aphid species was higher than within one species. Analysis of the mitochondrial haplotype ratio can be used to assess the influence of various factors, such as host plant resistance, on the structure of insect populations. A significant difference in the ratio of Sg1–Sg3 haplotypes was observed between Sch. graminum samples collected simultaneously on sorghum varieties differing in resistance to this aphid, and a unique Sg4 haplotype was found in the sample from the moderately resistant Efremovskoe Beloe variety.