Details

Autor(en) / Beteiligte

• González‐Cabrera, Jaime
• Sánchez‐González, Jorge Antonio
• Vázquez‐de‐Jesús, Fidel
• Mendoza‐Ceballos, Mario Yamill
• Muñiz‐Paredes, Facundo Rafael
• Arredondo‐Bernal, Hugo César

Titel

Inhibition of the spoilage yeast Pichia occidentalis in a wheat germ diet for mass rearing of Drosophila suzukii

Ist Teil von

• Entomologia experimentalis et applicata, 2023-01, Vol.171 (1), p.73-79

Ort / Verlag

Amsterdam: Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Quelle

Wiley Online Library

Beschreibungen/Notizen

• A wheat germ diet was initiated for the mass rearing of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) and subsequent production of its parasitoid Trichopria drosophilae Perkins (Hymenoptera: Diapriidae). However, the main problem of the diet was the microbial contamination affecting the batches of good pupae production. Therefore, the concentration of four antimicrobials was optimized through the Taguchi orthogonal array for the inhibition of microbial contamination, identified as Pichia occidentalis (Kurtzman et al.) Kurtzman et al. The optimized Taguchi‐selected‐antimicrobials concentrations were 1.33 g l−1 of potassium sorbate, 1.33 g l−1 of propyl paraben, 1.33 g l−1 of sodium propionate, and 0.16 g l−1 of triclosan. When the antimicrobials were added to the diet, colony‐forming units of P. occidentalis were inhibited by two orders of magnitude. Such inhibition means that the D. suzukii females produced 61.1 and 79.3% more pupae and adults, respectively, than the diet with no antimicrobials. These results increase the potential of the wheat germ diet as an artificial diet for mass rearing of D. suzukii and T. drosophilae. The rearing of Drosophila suzukii (Diptera: Drosophilidae) in a wheat germ diet has shown promising results. However, yeast contamination identified as Pichia occidentalis affects pupae production in the diet. Four antimicrobial concentrations were optimized for the inhibition of P. occidentalis, improving pupae and adult production by 61.1 and 79.3%, respectively. These results increase the potential of the wheat germ diet for D. suzukii pupae production and their use for the rearing of the parasitoid Trichopria drosophilae (Hymenoptera: Diapriidae).