Details

Autor(en) / Beteiligte

• Razak, Shahril Ab
• Azman, Nor Helwa Ezzah Nor
• Majid, Alny Marlynni Abd
• Kamarudin, Mohamad Zulfadli
• Rahman, Site Noorzuraini Abd
• Kamaruzaman, Rahiniza
• Zainal-Abidin, Rabiatul-Adawiah
• Abu-Bakar, Norliza

Titel

Identification of aromatic rice from genetic landrace resource using molecular marker integrated with chemical assessment

Ist Teil von

• Journal of biotech research, 2022-01, Vol.13, p.199-206

Ort / Verlag

Edmond: Bio Tech System

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• The price of aromatic rice is higher than the price of non-aromatic rice due to its aroma, which is the most integral characteristic of rice. The previous molecular analysis identified the deletion of an eight base-pairs (8-bp) region in exon 7 as the contributor to the aroma of most rice varieties, including Basmati and Jasmine rice. Therefore, the current study aimed to screen a set of Malaysian landrace rice with the use of a functional marker targeting the 8-bp deletion integrated with sensory assessment using KOH solution. Aromatic rice varieties exhibit a copy of the gene with the deletion of an eight base-pairs (8-bp), resulting in a frame shift mutation that hinders the enzyme activity of betaine aldehyde dehydrogenase (BADH2). As the aroma characteristic was expressed in a recessive manner, sensory assessment using KOH solution was performed on accessions with fgr/fgr allele only. The screening of total 186 rice accessions using functional markers revealed the following results: (1) 24 landrace rice accessions showed fgr/fgr (12.9%); (2) 149 landrace accessions showed Fgr/Fgr (80.1%); (3) 13 landrace rice accessions showed Fgr/fgr (7.0%). As for the chemical assessment using KOH solution, all 24 accessions with fgr/fgr allele expressed their aromatic sensory in the leaf aromatic test and grain aromatic test. However, the remaining accessions with Fgr/Fgr and Fgr/fgr may be aromatic due to the presence of other genes that control the aroma of rice. Hence, exploring the variability of the landrace was deemed significant in the identification of novel donor to be used or enhanced in the breeding program through marker-assisted breeding. Conclusively, this study successfully proved the feasibility of integrating molecular marker approach and sensory assessment for the mass screening of fgr/fgr allele in identifying aromatic rice from large germplasm collection and breeding program.