Details

Autor(en) / Beteiligte

• CHEN, Feng
• Xiao-Hua, ZENG
• Bin-Qiang, ZHANG
• LI, Bin
• Yan-Jiao, WANG
• Qin-Hua, CHEN
• Li-Na, KE

Titel

Effects and mechanism of RPDPC on proliferation and apoptosis of cervical cancer HeLa cells

Ist Teil von

• Jie fang jun yi xue za zhi, 2022-01, Vol.47 (9), p.879

Ort / Verlag

Beijing: People's Military Medical Press

Erscheinungsjahr

2022

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Objective　To investigate the effect of Rhopaladins' analogs RPDPC on cell biological properties of human cervical cancer HeLa cells and miR-21-5P/PTEN/PI3K signaling pathway. Methods　CCK-8 assay was used to detect the effects of different concentrations of RPDPC on the cell viabilities of HeLa cells treated for different time. DMSO (control), 12.5 μmol/L, 25.0 μmol/L and 50.0 μmol/L RPDPC were selected to treat HeLa cells for 48 h. Hoechst 33258 staining and Annexin-FITC/PI double staining were used to detect the effect of RPDPC on HeLa cell apoptosis; The expression levels of miR-21 and mRNA of E6, E7, PTEN, PI3K, Akt were detected by qRT-PCR; The expression levels of PTEN, p-PI3K, p-PI3K, p-Akt and Akt were detected by immunoblotting (WB). Results　Compared with the control group, viabilities of HeLa cells decreased significantly treated by RPDPC in different concentration and time (P<0.05); The apoptosis rate of HeLa cells tended to increase with increasing concentration of RPDPC (P<0.05); qRT-PCR results s