Details

Autor(en) / Beteiligte

• Xiao, Xiaoyue
• Huang, Yanmei
• Zhao, Xuelong
• Bao, Huanhuan
• Lu, Zhongwei
• Shan, Shan
• Liu, Daofeng
• Lai, Weihua

Titel

Intelligently identifiable membrane immunochip sensor based on Braille-like code for simultaneous multi-veterinary drug detection

Ist Teil von

• Sensors and actuators. B, Chemical, 2022-05, Vol.359, p.131571, Article 131571

Ort / Verlag

Lausanne: Elsevier B.V

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• There may be risk of multiple veterinary drug residues in one food sample; thus, a key challenge for the simultaneous detection of multiple targets was raised in food safety analysis. In this study, a multi-dot membrane immunochip sensor (MIS) based on Braille-like code was proposed for the sensitive, specific, and simultaneous detection of four veterinary drugs: sulfamethazine (SMZ), amantadine (AMD), danfloxacin (DAN), and tylosin (TYL). The 16 types of residual situations for four veterinary drugs can be qualitatively and quantitatively analyzed through the Braille-like code and signal intensity of dots. In addition, an Android application was exploited to intelligently identify the Braille-like code pattern; thus, the residue situations of the corresponding veterinary drugs can be automatically output by a smartphone. The cutoff values were 25 ng/mL for the four veterinary drugs by the naked eye. The limits of detection for SMZ, AMD, DAN, and TYL were calculated as 0.22, 0.058, 0.29, and 0.14 ng/mL, respectively. To verify the performance of this multi-dot MIS, spiked chicken samples with different concentrations were tested, and the results verified the accuracy and precision of this method. The average spiked recoveries were 79.2–116.7%, and the coefficient of variation was 1.6–10.5%. Flexible, versatile and intelligent discriminant methods give this multi-dot MIS great potential to rapidly recognize complex situations in the field of simultaneous detection of analytes. •A novel immunochip based on Braille code was proposed for simultaneous detection.•The type and quantity of residues could be intelligently identified by only one test.•Four veterinary drugs were quantified with high sensitivity and specificity.