Details

Autor(en) / Beteiligte

• Niu, Chun-dong
• Shi, Huan-ran
• Zhang, Zi-tong
• Fan, Tian-le
• Dang, Huan
• Chen, Peng-xiang
• Xie, Yin-peng
• Bao, Cha-na
• Ma, Feng-wang
• Guan, Qing-mei
• Li, Xue-wei

Titel

MdMYB88/124 modulates apple tree microRNA biogenesis through post-transcription processing and/or transcription pathway

Ist Teil von

• Acta physiologiae plantarum, 2022-08, Vol.44 (8), Article 86

Ort / Verlag

Berlin/Heidelberg: Springer Berlin Heidelberg

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• MicroRNAs are a class of endogenous small-RNA, which repress gene expression through post-transcriptional regulation. MdMYB88/124 (MYELOBLASTOSIS88/124), an R2R3-MYB transcription factor, regulates plants tolerance towards abiotic stress. In this study, we show that MdMYB88/124 alters the expression levels of some miRNAs, including mdm-miR156a-P5, mdm-miR164c-P5, mdm-miR166c-P5, mdm-miR391-P5, mdm-miR399-P3, mdm-miR399b-P5, mdm-miR2111a-P3, and mdm-miR7121a-P5. Furthermore, from the 18 analyzed pri-miRNAs, all of them showed increased expression in MdMYB88/124 RNAi plants. Y2H, BIFC, Split Luc, and Co-IP assays showed that MdMYB88/124 interacted with MdHYL1 (HYPONASTIC LEAVES1), a key factor in pri-miRNA and pre-miRNA splicing in vivo and in vitro. And we also found MdMYB88 could regulate the expression of MIR genes through the transcription pathway by dual-luciferase assay. In conclusion, we hypothesize that MdMYB88/124 can not only regulate MIR s expression by binding to their promoters, but also act as a co-factor with MdHYL1 to participate in miRNA biogenesis processing.