Details

Autor(en) / Beteiligte

• Wang, Shan
• Hu, Zunfu
• Wei, Qiulian
• Zhang, Huimin
• Tang, Weina
• Sun, Yunqiang
• Duan, Haiqiang
• Dai, Zhichao
• Liu, Qingyun
• Zheng, Xiuwen

Titel

Diatomic active sites nanozymes: Enhanced peroxidase-like activity for dopamine and intracellular H2O2 detection

Ist Teil von

• Nano research, 2022-05, Vol.15 (5), p.4266-4273

Ort / Verlag

Beijing: Tsinghua University Press

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Considering intracellular hydrogen peroxide (H 2 O 2 ) plays pivotal roles in the regulation of serial biological processes, the in-situ detection of intracellular H 2 O 2 has attracted an extensive attention. In the present work, an atomically dispersed diatomic active sites Nanozymes (FeN 3 /PtN 4 -single-atom nanozymes (SAzyme)) was prepared exhibiting enhanced peroxidase-like activity. The obvious synergistic effect between Fe-Pt heteronuclear diatomic active sites was confirmed by series of characterization and density functional theory (DFT). The peroxidase-like activity of Fe-sites could be substantially enhanced by the bonded Pt-sites via the modulation effect. As a consequence, the gap between the d-band centre ( ε d ) of Fe 3d orbitals and the Fermi energy level was narrowed and the electronic interaction could be strengthened, leading to a lower free energy barrier and a lower activation energy as well as fortified metal–O bonding in the kinetic pathway. Therefore, the constructed FeN 3 /PtN 4 -SAzyme exhibited higher peroxidase-like activity than that of FeN 4 -SAzyme. The FeN 3 /PtN 4 -SAzyme-assisted oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) facilitated the colorimetric detection of dopamine (DA), an important biomolecule. The linear detection range and limit of detection (LOD) of DA and H 2 O 2 were 1–10 µM, 0.01–1.0 mM and 0.109 µM, 7.97 µM, respectively. In addition, the constructed SAzymes were also applied for the in-situ detection of intracellular H 2 O 2 , expanding the application scope of the newborn SAzymes.