Details

Autor(en) / Beteiligte

• dos Santos Botelho, Deila Magna
• de Resende, Mário Lúcio Vilela
• Teixeira, Alexandre Rezende
• Santiago, Wilder Douglas
• Pozza, Edson Ampélio
• Moreira, Silvino Intra
• Ramos, Juliana Barros
• de Aquino, Sinara Oliveira
• Ferreira, Antônio Gilberto
• Reichel, Tharyn
• Guerra-Guimarães, Leonor

Titel

Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

Ist Teil von

• Chemical papers, 2022-04, Vol.76 (4), p.2567-2572

Ort / Verlag

Warsaw: Versita

Erscheinungsjahr

2022

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production.